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SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 Coronavirus

  • Catalog # : RAB01068-M01J
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  • Specification
  • Product Description:
  • Recombinant human monoclonal antibody raised against SARS-CoV-2 S.
  • Immunogen:
  • Recombinant protein corresponding to SARS-CoV-2 S.
  • Reactivity:
  • SARS-CoV-2
  • Specificity:
  • Reacts with RBD domain of spike protein of SARS-CoV-2, including B.1.1.7, B.1.351 and B.1.1.28.1 variants.
  • Form:
  • Liquid
  • Purification:
  • Protein A sepharose
  • Isotype:
  • IgG1
  • Storage Buffer:
  • In PBS, pH 7.4.
  • Storage Instruction:
  • Store at -80°C.
    Aliquot to avoid repeated freezing and thawing.
  • Applications
  • Flow Cytometry (Transfected cell)
  • Flow Cytometry (Transfected cell)
  • Flow cytometry (transfected cell) analysis of 293T-SARS-CoV-2 spike cells were treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J). This antibody efficiently bound to 293T- SARS-CoV-2 spike cells.
  • Inhibition Assay
  • Inhibition Assay
  • Protein receptor-binding inhibition assay of spike-His protein was treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J), and then added into 293T-ACE2 cells. Data were analyzed by flow cytometer. Binding affinity of spike-His protein to ACE2 is totally blocked by this antibody.
  • Neutralization
  • Neutralization
  • Neutralization analysis of pseudovirus was treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J), and then added into 293T-ACE2 cells. Luciferase activity was measured 48 hours post infection.
  • Neutralization
  • Neutralization
  • Neutralization analysis of variant pseudovirus was treated with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J), and then added into 293T-ACE2 cells. Luciferase activity was measured 48 hour post infection.
  • Neutralization
  • Neutralization
  • Cytopathic effect (CPE) based live virus neutralization analysis with human IgG1 isotype control and SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J). The antibody solutions (100 uL) were mixed in 1:1 (v/v) with suspension containing 200 TCID50 of SARS-CoV-2 virus.
  • Antibody Kinetics
  • Antibody Kinetics
  • Antibody kinetics with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J) and data were analyzed by biacore surface plasmon resonance.
  • Epitope Mapping
  • SARS-CoV-2 S1+S2 domain (residues 16-1213): positive
    SASRS-CoV-2 S1 domain (residues 1-674): positive
    SARS-CoV-2 receptor binding domain (residues 319-541): positive
    SARS-CoV-2 core domain + receptor binding subdomain (residues 387-516): positive
    SARS-CoV-2 receptor-binding motif (residues 438-505): positive
    Data were analyzed by indirect ELISA.
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
  • ELISA analysis coated mucin (100 ug/mL) with SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J).
  • Neutralization
  • Neutralization
  • In in vivo neutralization analysis, hamsters received 2 doses of SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J) by intraperitoneal injection and then were infected with 3 doses pseudovirus through intanasal injection. Lung tissues were collected one day post last dose pseudovirus treatment. Luciferase gene expression level was detected by QPCR.
  • Neutralization
  • Neutralization
  • In in vivo nasal prophylaxis neutralization analysis, hamsters were infected with B.1.1.28.1 variant spike pseudovirus 2 hours after receiving SARS-CoV-2 S recombinant human monoclonal antibody, clone 7F7 (Cat # RAB01068-M01J) for three times. Lung tissues were collected one day post last dose pseudovirus treatment. Luciferase gene expression level was detected by QPCR.
  • Application Image
  • Flow Cytometry (Transfected cell)
  • Flow Cytometry (Transfected cell)
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  • Epitope Mapping
  • Enzyme-linked Immunoabsorbent Assay
  • Enzyme-linked Immunoabsorbent Assay
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